Differences in QNB binding between freshly isolated and cultured single cells prepared from longitudinal smooth muscle of guinea pig ileum
نویسندگان
چکیده
منابع مشابه
Heterogeneous contractile responses of single smooth muscle cells from guinea pig ileum.
Cells of guinea pig ileal longitudinal muscle layer were dispersed by treating the minced tissue with highly purified collagenase and papain. The cells were spindle shaped, 100 to 150 microns long and 5 to 10 microns wide. Contractility of the dispersed cells was determined by "cell adhesion method" resulting that the cells were contracted by carbachol in a dose-dependent manner, but sensitivit...
متن کاملSpontaneous oscillations of cytoplasmic free calcium ion concentration in cultured smooth muscle cells from guinea pig ileum.
The cytoplasmic free calcium ion concentration ([Ca2+]i) of cultured guinea pig ileum longitudinal muscle cells loaded with a fluorescent [Ca2+]i indicator, fura-2, was measured by digital ratio imaging microscopy. Spontaneous [Ca2+]i oscillations were observed in 25% to 80% of the cells, which differed with the batches of the cultured cells after 5 to 8 days in culture. The frequency and ampli...
متن کاملSmooth muscle relaxing drugs and guinea pig ileum.
The effects of various smooth muscle relaxing drugs on contractile responses to acetylcholine (ACh), Ba2+ and Ca2+, and on the tissue cyclic AMP levels were examined in the guinea pig ileum. Papaverine and theophylline caused a decrease both in the maximum height and the slope of dose-response curves induced by the three stimulants, and an increase in the cyclic AMP levels. Diltiazem and D-600 ...
متن کاملNexuses between the smooth muscle cells of the guinea-pig ileum
The circular musculature of the guinea-pig ileum has been studied by freeze-fracture to analyze quantitatively the gap junctions (nexuses) between its smooth muscle cells. The average cell surface area and cell volume are 5,074 micron 2 and 3,260 micron 3. The packing density of nexuses is 48/1,000 micron 2 of cell surface or approximately 244/muscle cell. Nexuses range in area from less than 0...
متن کاملMuscarinic cholinergic stimulation of phosphatidylinositol turnover in the longitudinal smooth muscle of guinea-pig ileum.
1. The metabolism of phosphatidylinositol and phosphatidate was investigated in fragments of longitudinal smooth muscle from guinea-pig ileum incubated with cholinergic and anticholinergic drugs. 2. Incorporation of Pi into these lipids was enhanced by acetylcholine and carbamoylcholine. 3. The receptor responsible for triggering this response was of the muscarinic type, since (a) the response ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1989
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)56682-3